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The next day, samples were incubated with FITC-conjugated, TRITC-conjugated, or Cy5-conjugated (1:1000, Invitrogen, United States) secondary antibody along with 4,6-diamidino-2-phenylindole (DAPI) (1:1000, Sigma-Aldrich, United States) at room temperature for 1 h

As has been discovered in numerous epidemiological and experimental investigations, these metals have also been categorised as human carcinogens by the U.S

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To be safe, you always need to dilute the mix before employing onto your own face