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Briefly, 400 m slices were cultured on (Milipore, Billerica, Mass.) for 3 days after which they were biolistically transfected with tomato fluorescent protein (TFP) using a Helios Gen Gun (BioRad, Hercules, Calif.), according to the manufacturer's protocol, to visualize dendritic arbors

doi: 10.1007/s00240-010-0265-6

Any yellow, brown, or pink tint indicates chemical degradation